Abstract
Solid organ transplant recipients are vulnerable to severe infection during induction therapy. We report a case of a 67-year-old male who died unexpectedly 10 days after receiving a kidney transplant on February 10, 2020. There was no clear cause of death, but COVID-19 was considered retrospectively, as the death occurred shortly after the first confirmed case of COVID-19 in Canada. We confirmed the presence of SARS-CoV-2 components in the renal allograft and native lung tissue using immunohistochemistry for SARS-CoV-2 spike protein and RNA scope in situ hybridization for SARS-CoV-2 RNA. Results were reaffirmed with the Food and Drug Administration Emergency Use Authorization approved Bio-Rad SARS-CoV-2 digital droplet PCR for the kidney specimen. Our case highlights the importance of patient autopsies in an unfolding global pandemic and demonstrates the utility of molecular assays to diagnose SARS-CoV-2 post-mortem. SARS-CoV-2 infection during induction therapy may portend a fatal clinical outcome. We also suggest COVID-19 may be transmittable via renal transplant.
Keywords: basic (laboratory) research/science; clinical research/practice; donors and donation: donor-derived infections; infection and infectious agents-viral; kidney transplantation/nephrology; kidney transplantation: living donor; pathology/histopathology; patient safety.
【저자키워드】 patient safety, clinical research/practice, kidney transplantation/nephrology, kidney transplantation: living donor, infection and infectious agents-viral, basic (laboratory) research/science, donors and donation: donor-derived infections, pathology/histopathology, 【초록키워드】 COVID-19, SARS-CoV-2, immunohistochemistry, Severe infection, therapy, SARS-COV-2 infection, Infection, Organ transplant, drug, Autopsy, patient safety, Laboratory, RNA, Clinical outcome, kidney, global pandemic, PCR, SARS-CoV-2 spike protein, male, Patient, death, Post-mortem, SARS-CoV-2 RNA, utility, Canada, Molecular assay, kidney transplant, diagnose, Donor, food, Emergency, Allograft, specimen, lung tissue, component, renal, authorization, recipient, Bio-rad, highlight, Result, occurred, died, approved, receiving, Solid, 【제목키워드】 SARS-CoV-2, death, molecular, kidney transplant,