Acute respiratory tract infections (ARTIs) are among the leading causes of morbidity and mortality in children. The viral etiology of ARTIs was investigated over 3 years (October 2012–September 2015) in 2575 children in Parma, Italy, using indirect immunofluorescent staining of respiratory samples for viral antigens, cell culture, and molecular assays. Respiratory viruses were detected in 1299 cases (50.44%); 1037 (79.83%) were single infections and 262 (20.17%) mixed infections. The highest infection incidence was in children aged >6 months to ≤3 years (57.36%). Human respiratory syncytial virus (27.12%) and human adenovirus (23.58%) were the most common viruses identified. The virus detection rate decreased significantly between the first and third epidemic season (53.9% vs. 43.05%, P < 0.0001). The simultaneous use of different diagnostic tools allowed us to identify a putative viral etiology in half the children examined and to provide an estimate of the epidemiology and seasonality of respiratory viruses associated with ARTIs. Highlights • Respiratory viruses were assessed in children from October 2012 to September 2015. • Viruses were detected using antigen and molecular assays, and cell culture. • Respiratory syncytial virus and adenovirus were the most common viruses detected. • Influenza virus and respiratory syncytial virus detection showed seasonal variation. • Respiratory virus detection was highest in children aged >6 months to ≤3 years.
【저자키워드】 Epidemiology, respiratory viruses, laboratory diagnosis, BSA, bovine serum albumin, CPE, cytopathic effect, HCoV, human coronavirus, PCR, polymerase chain reaction, molecular assays, IV, influenza virus, ARTI, acute respiratory tract infection, mAb, monoclonal antibody, Acute respiratory tract infections, HBoV, human bocavirus, PBS, phosphate-buffered saline, hMPV, human metapneumovirus, NPA, nasopharyngeal aspirate, HAdV, Human adenovirus, HEV, human enterovirus, BAL, bronchial alveolar lavage, BAS, bronchial aspirate, CV, coxsackievirus, ECHO, echovirus, HEP-2, human larynx epidermoid carcinoma, HPIV, human parainfluenza virus, HRSV, human respiratory syncytial virus, IAV, influenza virus A, IBV, influenza virus B, IIF, indirect immunofluorescence assay, Intestine 407, embryonic human intestine, LLC-MK2, rhesus monkey-kidney, MDCK-SIAT1, Madin-Darby Canine Kidney with enhanced expression of 6-linked sialic acids, MRC-5, human lung fibroblasts, NS, nasal swab, RT-PCR, real-time PCR, rt-RT-PCR, reverse transcription RT-PCR, SP, sputum, TS, throat swab, Vero, African green monkey kidney, Virus isolation in cell culture,