An outbreak of betacoronavirus SARS-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR-Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from US patients, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US CDC SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.. Editor’s summary SARS-CoV-2 in patient samples is detected in under an hour using a CRISPR-based lateral flow assay.
【초록키워드】 COVID-19, SARS-CoV-2, Infection, Betacoronavirus, global pandemic, lateral flow, outbreak, Patient, Swab, real-time RT-PCR, patients, Predictive, viral respiratory infections, positive, US CDC, Wuhan, China, RNA extracts, clinical sample, spread to, the disease, provide, faster, patients with COVID-19, 【제목키워드】 SARS-CoV-2,