Highlights • RT-LAMP test kits have good utility. • Detection sensitivity of 1.0 × 10 1 copies/μL can be obtained within 35 min. • RT-LAMP can be used as a point-of-care test because it is judged by turbidity under natural light. With the rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is an urgent need for more rapid and simple detection technologies at the forefront of medical care worldwide. In this study, we evaluated the effectiveness of the Loopamp® 2019-SARSCoV-2 Detection Reagent Kit, which uses loop-mediated isothermal amplification (LAMP) technology. In this protocol, cDNA is synthesized from SARS-CoV-2 RNA using reverse transcriptase, followed by DNA amplification under isothermal conditions in one step. The RT-LAMP test kit amplified the targeted RNA of a SARS-CoV-2 isolate with a detection limit of 1.0 × 101 copies/μL, which was comparable to the detection sensitivity of quantitative reverse transcription PCR (RT-qPCR). Comparison with the results of RT-qPCR for 76 nasopharyngeal swab samples from patients with suspected COVID-19 showed a sensitivity of 100 % and a specificity of 97.6 %. In the 24 RNA specimens derived from febrile Japanese patients with or without influenza A, no amplification was observed using RT-LAMP. RT-LAMP could be a simple and easy-to-use diagnostic tool for the detection of SARS-CoV-2.
【저자키워드】 COVID-19, SARS-CoV-2, RT-qPCR, LAMP, 【초록키워드】 coronavirus, protocol, diagnostic, detection, influenza A, RNA, Spread, point-of-care, amplification, RT-LAMP, sensitivity, specificity, Patient, Effectiveness, isothermal amplification, SARS-CoV-2 RNA, Japanese, utility, reverse transcription PCR, Quantitative, Care, Detection sensitivity, Detection limit, isothermal, cDNA, acute respiratory syndrome, specimen, reagent, nasopharyngeal swab sample, transcriptase, febrile, DNA amplification, amplified, evaluated, can be used, condition, comparable, 【제목키워드】 Diagnosis, novel coronavirus disease, isothermal amplification,