The antibacterial activity of water-soluble chitosan derivatives prepared by Maillard reactions against Staphylococcus aureus , Listeria monocytogenes , Bacillus cereus , Escherichia coli , Shigella dysenteriae , and Salmonella typhimurium was examined. Relatively high antibacterial activity against various microorganisms was noted for the chitosan-glucosamine derivative as compared to the acid-soluble chitosan. In addition, it was found that the susceptibility of the test organisms to the water-soluble chitosan derivative was higher in deionized water than in saline solution. Metal ions were also found to reduce the antibacterial activity of the water-soluble chitosan derivative on S. aureus . The marked increase in glucose level, protein content and lactate dehydrogenase (LDH) activity was observed in the cell supernatant of S. aureus exposed to the water-soluble chitosan derivative in deionized water. The results suggest that the water-soluble chitosan produced by Maillard reaction may be a promising commercial substitute for acid-soluble chitosan.
【저자키워드】 chitosan, Maillard reaction, antibacterial activity, water-soluble chitosan,