A novel coronavirus, named SARS-CoV-2, emerged in 2019 in China and rapidly spread worldwide. As no approved therapeutics exists to treat COVID-19, the disease associated to SARS-Cov-2, there is an urgent need to propose molecules that could quickly enter into clinics. Repurposing of approved drugs is a strategy that can bypass the time-consuming stages of drug development. In this study, we screened the PRESTWICK CHEMICAL LIBRARY composed of 1,520 approved drugs in an infected cell-based assay. The robustness of the screen was assessed by the identification of drugs that already demonstrated in vitro antiviral effect against SARS-CoV-2. Thereby, 90 compounds were identified as positive hits from the screen and were grouped according to their chemical composition and their known therapeutic effect. Then EC50 and CC50 were determined for a subset of 15 compounds from a panel of 23 selected drugs covering the different groups. Eleven compounds such as macrolides antibiotics, proton pump inhibitors, antiarrhythmic agents or CNS drugs emerged showing antiviral potency with 2 < EC50 ≤ 20 µM. By providing new information on molecules inhibiting SARS-CoV-2 replication in vitro, this study provides information for the selection of drugs to be further validated in vivo. Disclaimer: This study corresponds to the early stages of antiviral development and the results do not support by themselves the use of the selected drugs to treat SARS-CoV-2 infection.
【저자키워드】 SARS-CoV-2, Drug discovery, 【초록키워드】 COVID-19, Antiviral, SARS-COV-2 infection, Antibiotics, repurposing, drug, in vitro, inhibitors, Novel coronavirus, Antiviral effect, clinics, Spread, China, in vivo, information, CNS, early stage, SARS-CoV-2 replication, Support, approved drug, Compound, Stage, therapeutic effect, treat, positive, antiviral potency, EC50, composed, the disease, approved, screened, provide, demonstrated, groups, subset, inhibiting, time-consuming, selected drug, 【제목키워드】 FDA, Replication, inhibitors of SARS-CoV-2, approved, reveal,