Ongoing pandemic and potential resurgence of Coronavirus disease 2019 (COVID-19) has prompted urgent efforts to investigate the immunological memory of convalescent patients, especially in patients with active cancers. Here we performed single-cell RNA sequencing in peripheral blood samples of 3 healthy donors (HDs), 4 COVID-19 patients (Covs) and 4 COVID-19 patients with active gynecological tumor (TCs) pre- and post- anti-tumor treatment. All Covs patients had recovered from their acute infection. Interestingly, the molecular features of PBMCs in TCs are similar to that in Covs, suggesting that convalescent COVID-19 with gynecologic tumors do not have major immunological changes and may be protected against reinfection similar to COVID-19 patients without tumors. Moreover, the chemotherapy given to these patients mainly caused neutropenia, while having little effect on the proportion and functional phenotype of T and B cells, and T cell clonal expansion. Notably, anti-PD-L1 treatment massively increased cytotoxic scores of NK cells, and T cells, and facilitated clonal expansion of T cells in these patients. It is likely that T cells could protect patients from SARS-CoV-2 virus reinfection and anti-PD-L1 treatment can enhance the anti-viral activity of the T cells.
【저자키워드】 COVID-19, Tumor, Chemotherapy, ICIs, single cell sequencing, 【초록키워드】 Treatment, coronavirus disease, Tumor, Coronavirus disease 2019, pandemic, T cells, B cells, NK cells, SARS-CoV-2 virus, Immunological memory, Single-cell RNA sequencing, Peripheral blood, Chemotherapy, Reinfection, T cell, Convalescent patients, cancers, acute infection, Patient, PBMC, anti-viral activity, convalescent, patients, COVID-19 patients, neutropenia, PBMCs, T and B cells, COVID-19 patient, tumors, clonal expansion, CoVs, effort, peripheral blood samples, functional phenotype, molecular features, resurgence, healthy donor, ENhance, PROTECT, performed, caused, proportion, facilitated, immunological change, molecular feature, 【제목키워드】 Immunotherapy, Impact, convalescent, Immune cell,