The biofilm forming behavior of 51 Salmonella Typhimurium strains was determined in Tryptone Soya Broth (TSB) and 20 times diluted TSB (1/20TSB) at 25°C and 37°C. The results indicated that biofilm forming behavior is influenced by environmental conditions and associated with the origin of the strains. Clinical, outbreak-associated and retail product isolates showed dense biofilm formation in both media at 25°C, and in TSB also at 37°C. However, industrial isolates only showed dense biofilm formation in 1/20TSB at 25°C. By enumeration of biofilm cells, LIVE/DEAD staining and SEM analysis of biofilms it was found that the ratio of cells and extracellular matrix is affected by environmental conditions. Indeed, the genes involved in curli fimbriae and cellulose production are highly induced during biofilm formation at 25°C in 1/20TSB. This indicates that these are important matrix components during biofilm formation in 1/20TSB at 25°C and that other factors contribute to biofilm formation of clinical, outbreak-associated and retail product isolates at 37°C and/or nutrient-rich conditions.
Diversity in biofilm formation and production of curli fimbriae and cellulose ofSalmonellaTyphimurium strains of different origin in high and low nutrient medium
다양성이 높은 영양 매질과 저영양 매질에서 다양한 원산지의 살모넬라 타이푸리움 균주의 바이오필름 형성 및 컬리 피브리아 및 섬유소 생산.
[Category] 살모넬라증,
[Article Type] journal-article
[Source] pubmed
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