The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE’s results can be visualized with an in-tube fluorescent readout — reducing contamination risk as amplification reaction tubes remain sealed — and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities. The COVID-19 pandemic has highlighted the need for user-friendly diagnostic techniques. Here, the authors present SHINE, a streamlined and optimised Cas13-based method with accompanying smartphone app for visual diagnosis.
【저자키워드】 Assay systems, Infectious-disease diagnostics, CRISPR-Cas systems, Molecular engineering, 【초록키워드】 Saliva, COVID-19 pandemic, hospital, diagnostic, Diagnosis, risk, virus, Nasopharyngeal swab, amplification, sensitivity, specificity, RT-qPCR, Contamination, nasopharyngeal, Patient, SARS-CoV-2 RNA, disease transmission, diagnostic capabilities, fluorescent, IMPROVE, identify, detect, develop, condition, occur, reducing, sealed, reaction tube, 【제목키워드】 SARS-CoV-2, amplification, inactivation,