Patients with chronic lung disease (CLD) have an increased risk for severe coronavirus disease-19 (COVID-19) and poor outcomes. Here, we analyze the transcriptomes of 611,398 single cells isolated from healthy and CLD lungs to identify molecular characteristics of lung cells that may account for worse COVID-19 outcomes in patients with chronic lung diseases. We observe a similar cellular distribution and relative expression of SARS-CoV-2 entry factors in control and CLD lungs. CLD AT2 cells express higher levels of genes linked directly to the efficiency of viral replication and the innate immune response. Additionally, we identify basal differences in inflammatory gene expression programs that highlight how CLD alters the inflammatory microenvironment encountered upon viral exposure to the peripheral lung. Our study indicates that CLD is accompanied by changes in cell-type-specific gene expression programs that prime the lung epithelium for and influence the innate and adaptive immune responses to SARS-CoV-2 infection. Patients with chronic lung disease (CLD) have an increased risk for severe coronavirus disease-19 and poor outcomes. Here the authors compare the transcriptomes of single cells isolated from healthy and CLD lungs to identify molecular characteristics of lung cells that may account for worse COVID-19 outcomes in these patients.
【저자키워드】 Inflammation, viral infection, Computational biology and bioinformatics, Immunopathogenesis, 【초록키워드】 COVID-19, Transcriptome, Gene Expression, innate immune response, SARS-COV-2 infection, lung, Lung disease, outcome, outcomes, Characteristics, Coronavirus disease-19, viral replication, Lungs, Patient, Single Cell, molecular, Adaptive immune response, patients, AT2, Inflammatory, SARS-CoV-2 entry factor, Efficiency, chronic lung diseases, CLD, increased risk, relative expression, lung epithelium, Express, microenvironment, cellular distribution, Alter, Cell, lung cell, highlight, observé, identify, healthy, indicate, changes in, accompanied, 【제목키워드】 Gene Expression, severity, chronic lung disease, SARS-CoV-2 entry,