Middle East respiratory syndrome coronavirus (MERS-CoV) is enzootic in dromedary camels across the Middle East and Africa. Virus-induced pneumonia in humans results from animal contact, with a potential for limited onward transmission. Phenotypic changes have been suspected after a novel recombinant clade (lineage 5) caused large nosocomial outbreaks in Saudi Arabia and South Korea in 2016. However, there has been no functional assessment. Here we perform a comprehensive in vitro and ex vivo comparison of viruses from parental and recombinant virus lineages (lineage 3, n = 7; lineage 4, n = 8; lineage 5, n = 9 viruses) from Saudi Arabia, isolated immediately before and after the shift toward lineage 5. Replication of lineage 5 viruses is significantly increased. Transcriptional profiling finds reduced induction of immune genes IFNB1 , CCL5 , and IFNL1 in lung cells infected with lineage 5 strains. Phenotypic differences may be determined by IFN antagonism based on experiments using IFN receptor knock out and signaling inhibition. Additionally, lineage 5 is more resilient against IFN pre-treatment of Calu-3 cells (ca. 10-fold difference in replication). This phenotypic change associated with lineage 5 has remained undiscovered by viral sequence surveillance, but may be a relevant indicator of pandemic potential. MERS-CoV is enzootic in dromedary camels, can spread to humans but undergoes limited onward transmission. Here, Schroeder et al. compare clinical isolates of MERS-CoV in vitro and show that the predominantly circulating recombinant lineage 5 possess a fitness advantage over parental lineage 3 and 4 due to reduced activation of innate immune signaling.
【저자키워드】 viral epidemiology, viral evolution, Viral immune evasion, 【초록키워드】 viruses, pandemic, Pneumonia, Human, in vitro, virus, MERS-CoV, Saudi Arabia, Replication, Surveillance, Lineage, clade, nosocomial outbreak, IFN, receptor, experiment, Calu-3 cell, South Korea, change, Strains, Signaling, innate immune, Contact, Middle East, Ex vivo, Activation, sequence, CCL5, circulating, onward transmission, respiratory syndrome coronavirus, phenotypic, IFNB1, isolate, immune gene, lung cell, significantly increased, caused, spread to, remained, reduced, functional, parental, IFNL1, 【제목키워드】 Lineage, replicative fitness, reveal,