The rapid spread of coronavirus SARS-CoV-2 greatly threatens global public health but no prophylactic vaccine is available. Here, we report the generation of a replication-incompetent recombinant serotype 5 adenovirus, Ad5-S-nb2, carrying a codon-optimized gene encoding Spike protein (S). In mice and rhesus macaques, intramuscular injection with Ad5-S-nb2 elicits systemic S-specific antibody and cell-mediated immune (CMI) responses. Intranasal inoculation elicits both systemic and pulmonary antibody responses but weaker CMI response. At 30 days after a single vaccination with Ad5-S-nb2 either intramuscularly or intranasally, macaques are protected against SARS-CoV-2 challenge. A subsequent challenge reveals that macaques vaccinated with a 10-fold lower vaccine dosage (1 × 10 10 viral particles) are also protected, demonstrating the effectiveness of Ad5-S-nb2 and the possibility of offering more vaccine dosages within a shorter timeframe. Thus, Ad5-S-nb2 is a promising candidate vaccine and warrants further clinical evaluation. A vaccine protecting from SARS-CoV-2 infection is needed. Here the authors generate a replication-incompetent adenovirus based vaccine expressing SARS-CoV-2 spike, show protection from infection in non-human primates, and analyze the immune response after intramuscular and intranasal vaccination.
【저자키워드】 SARS-CoV-2, viral infection, Experimental models of disease, Live attenuated vaccines, 【초록키워드】 Vaccine, immune response, vaccination, spike, antibody, SARS-COV-2 infection, Antibody Response, Infection, Prophylactic, coronavirus SARS-CoV-2, Adenovirus, Spread, Protein, clinical evaluation, mice, non-human primates, Effectiveness, macaque, intranasal, intramuscular, SARS-CoV-2 spike, rhesus macaques, Viral particles, Intramuscular injection, serotype, global public health, dosage, candidate vaccine, gene encoding, single vaccination, CMI, responses, intranasally, subsequent, generate, elicit, reveal, expressing, cell-mediated immune, intramuscularly, 【제목키워드】 COVID-19 vaccine, rhesus macaque,