Highlights • Surface Plasmon Resonance imaging is an unprecedented technology for high throughput screening of antibody profiling of CoViD19 patients. • Fingerprinting of isotypes IgM, IgG and IgA can be performed for 384 patients in one run. • Severity of the disease correlates well with the total anti-RBD of SARS-CoV-2 concentration in CoViD19 patients • Affinity equilibrium constant (K D ) of the polyclonal antibody binding was directly proportional to the off-rate (k d ) simplifying the screening. • Screening of the strength of binding of anti RBD antibodies was possible in high throughput and in one run together with the isotype analysis in the LSA SPR imager. • An affinity maturation effect was shown for patients recovering from CoViD19. • A tool is now available to test the quality of the immune reaction of individuals to SARS-CoV-2 and its mutants in vaccination programs. Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding of IgG, IgM and IgA against the Receptor Binding Domain (RBD) of SARS-CoV-2 in sera of 102 CoViD-19 and non-CoViD-19 patients. The SPRi assay simultaneously measures the antibody isotype levels and the strength of binding to the RBD of ultimate 384 patient samples in one run. It turns out that during the course of the disease, the IgG levels and strength of binding increased while generally the IgM and IgA levels go down. Recovered patients all show high strength of binding of the IgG type to the RBD protein. The anti-RBD immunoglobulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19. This new high throughput method can be applied for the assessment of the quality of the immune reaction of healthy individuals to SARS-CoV-2 and its mutants in vaccination programs. • Surface Plasmon Resonance imaging is an unprecedented technology for high throughput screening of antibody profiling of CoViD19 patients. • Fingerprinting of isotypes IgM, IgG and IgA can be performed for 384 patients in one run. • An affinity maturation effect was shown for patients recovering from CoViD19. Graphical abstract Image, graphical abstract
【저자키워드】 Infectious diseases, biosensor, Corona, high throughput, antibody isotypes, affinity, Off-rate, 【초록키워드】 SARS-CoV-2, IgG, IgM, vaccination, antibody, SPR, Screening, Antibody binding, Immunoglobulin, RBD, IgA, sera, Patient, mutant, surface, recovered, patients, Affinity maturation, antibody profiling, binding, Immune status, Concentration, Analysis, Image, anti-RBD, Abstract, individual, isotype, measure, immune reaction, resonance, polyclonal, Course, shown, performed, was used, the disease, provide, determine, the RBD, turn, healthy individual, IgA level, IgG level, Plasmon, the RBD protein, 【제목키워드】 SARS-CoV-2, IgG, IgM, Infection, binding, IgA antibody,