We describe a rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the direct detection and quantitation of SARS-CoV-2 nucleoprotein in gargle solutions and saliva. The method is based on a multiple-reaction monitoring (MRM) mass spectrometry approach with a total cycle time of 5 min per analysis and allows the detection and accurate quantitation of SARS-CoV-2 nucleoprotein as low as 500 amol/μL. We improved the sample preparation protocol of our recent piloting SARS-CoV-2 LC-MS study regarding sensitivity, reproducibility, and compatibility with a complementary reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of the same sample. The aim of this work is to promote diagnostic tools that allow identifying and monitoring SARS-CoV-2 infections by LC-MS/MS methods in a routine clinical environment. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03614-y.
【저자키워드】 SARS-CoV-2, mass spectrometry, Liquid chromatography, Multiple-reaction monitoring, Triple quadrupole, 【초록키워드】 Saliva, mass spectrometry, protocol, SARS-COV-2 infection, diagnostic, polymerase chain reaction, sensitivity, RT-qPCR, Quantitative, SARS-CoV-2 infections, LC-MS, Tandem mass spectrometry, Analysis, complementary, reverse transcriptase, Chain Reaction, gargle solutions, Abstract, supplementary material, reproducibility, quantitation, SARS-CoV-2 nucleoprotein, compatibility, transcriptase, approach, polymerase chain, promote, piloting, 【제목키워드】 Analysis,