Insoluble immune-complexes (IC) are solubilized by complement. A functional alternative pathway (AP) is required for solubilization. Classical pathway (CP) has an enhancing effect, but the effect of antigen-antibody (Ag-Ab) ratio and the relative role of AP and CP at these different Ag-Ab ratios is controversial. We reinvestigated these factors in a human model (IC prepared with tetanus toxoid and affinity purified human Ab solubilized by normal serum–NHS). We present evidence that (1) IC prepared at Ag excess are only partially solubilized. (2) IC prepared at large Ag excess are not solubilized by AP. (3) AP prepared at Ab excess are solubilized exclusively by AP. (4) IC prepared at equivalence are solubilized by AP and CP acting in synergy.
Immune-complexes solubilization: Effect of antigen-antibody ratio and relative role of alternative and classical complement pathways
면역 복합체 용해: 항원-항체 비율의 효과와 대체 및 고전 보체 경로의 상대적 역할
[Category] 파상풍,
[Article Type] journal-article
[Source] pubmed
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