We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.
All Keywords
【저자키워드】 Multiplex PCR, sensitivity, specificity, pneumococci, Quellung reaction, Serogroup/type,
【저자키워드】 Multiplex PCR, sensitivity, specificity, pneumococci, Quellung reaction, Serogroup/type,