High-temperature requirement A (HtrA), a highly conserved family of serine protease, plays crucial roles in protein quality control in prokaryotes and eukaryotes. The HtrA protein contains a C-terminal PDZ domain that mediates the proteolytic activity. Here we reported the solution structure of the HtrA PDZ domain from Streptococcus pneumoniae by NMR spectroscopy. Our results showed that the structure of HtrA PDZ domain, which contains three α-helices and five β-strands, illustrates conservation within the canonical PDZ domains. In addition, we demonstrated the interactions between S. pneumoniae HtrA PDZ domain and peptides with the motif XXX-YYF-COOH by surface plasmon resonance. Besides, we identified the ligand binding surface and the critical residues responsible for ligand binding of HtrA PDZ domain by chemical shift perturbation and site-directed mutagenesis.
Solution structure of HtrA PDZ domain from Streptococcus pneumoniae and its interaction with YYF–COOH containing peptides
Streptococcus pneumoniae의 HtrA PDZ 도메인의 구조와 YYF–COOH를 포함하는 펩타이드와의 상호작용
[Category] 폐렴구균 감염증,
[Article Type] journal-article
[Source] pubmed
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