With the spread of new variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), there is a need to assess the protection conferred by both previous infections and current vaccination. Here we tested the neutralizing activity of infected and/or vaccinated individuals against pseudoviruses expressing the spike of the original SARS-CoV-2 isolate Wuhan-Hu-1 (WH1), the D614G mutant and the B.1.1.7 variant. Our data show that parameters of natural infection (time from infection and nature of the infecting variant) determined cross-neutralization. Uninfected vaccinees showed a small reduction in neutralization against the B.1.1.7 variant compared to both the WH1 strain and the D614G mutant. Interestingly, upon vaccination, previously infected individuals developed more robust neutralizing responses against B.1.1.7, suggesting that vaccines can boost the neutralization breadth conferred by natural infection.
【저자키워드】 SARS-CoV-2, neutralization, B.1.1.7 variant, Humoral response, pseudovirus, 【초록키워드】 Vaccine, coronavirus, vaccination, neutralization, variant, Infection, severe acute respiratory syndrome Coronavirus, Spread, Neutralizing activity, B.1.1.7 variant, pseudovirus, B.1.1.7, Wuhan, cross-neutralization, D614G, mutant, respiratory, natural infection, boost, pseudoviruses, acute respiratory syndrome, acute respiratory syndrome coronavirus, acute respiratory syndrome coronavirus 2, infected individual, vaccinated individuals, neutralization breadth, vaccinated individual, parameter, Wuhan-Hu-1, infecting, vaccinee, robust, tested, expressing, reduction in, neutralizing response, 【제목키워드】 individual, increase,