The evolution of vaccine product quality during batch cultivation of Bordetella pertussis, the causative agent of whooping cough, was investigated with the goal to determine the optimal harvest point. The process was explored by measuring mRNA expression at frequent intervals during cultivation. The genes that are involved in virulence are already known for this product and changes in their expression levels are proposed to be indicative for product quality. A quantitative product quality score is calculated based on the expression levels of these virulence genes, which allows comparison of expected product quality between culture samples. Product quality scores were maximal throughout the logarithmic growth phase, but dropped significantly at the start of the stationary phase. This showed that the decreasing lactate and glutamate concentrations towards the end of the batch are critical for product quality. On-line measurement of these nutrients allows the cultivation process to be harvested at the optimal harvest point, increasing process robustness and consistency.
Gene‐expression‐based quality scores indicate optimal harvest point in Bordetella pertussis cultivation for vaccine production
유전자 발현 기반 품질 점수는 백신 생산을 위한 Bordetella pertussis 배양의 최적 수확 시점을 나타냅니다.
[Category] 백일해,
[Article Type] journal-article
[Source] pubmed
All Keywords