Mechanism Of Reappearance Of Contact Sensitivity To Dncb In Desensitized Guinea Pigs

The different immunologic activities of lymph node and spleen cells as compared to peritoneal exudate cells were studied in temporarily desensitized, DNCB-contact-sensitive guinea pigs. Lymph node (and spleen) lymphocytes: (a) transfer contact sensitivity to syngeneic recipients when harvested at the time of unresponsiveness; (b) are only temporarily inactivated in tolerant recipients by the desensitizing procedure; (c) enter the lymph node (or spleen) compartments in a higher proportion than peritoneal exudate cells; (d) are not susceptible to inactivation in vitro; and (e) are not permanently desensitized when released into the circulation by an intravenous injection of Bordetella pertussis vaccine. These results indicate that the resistance of primed lymph node cells to the desensitizing procedure may account for the reappearance of contact sensitivity in desensitized guinea pigs.