The spinal cords of mice that were infected with the BeAn 8386 strain of Theiler’s murine encephalomyelitis virus (TMEV) were studied to elucidate the involvement of osteopontin in the course of TMEV-induced demyelination. Immunohistochemistry showed staining for osteopontin in the vessels of the normal spinal cords, and more intense immunoreactivity in the vessels within the demyelinating lesions. Intense osteopontin immunoreactivity was observed in the cell bodies, as well as in the extracellular space of the demyelinating lesions, where some glial cells, which included activated microglia/macrophages, were also immunopositive for osteopontin. These findings suggest that osteopontin is upregulated in the demyelinating spinal cord, and that osteopontin from either microglia or astrocytes may be involved in the chemotaxis of inflammatory cells and astrocytes, which ultimately leads to chronic inflammation and astrogliosis in this model system.
Immunohistochemical detection of osteopontin in the spinal cords of mice with Theiler’s murine encephalomyelitis virus-induced demyelinating disease
타일러의 생쥐 뇌척수염 바이러스에 의해 유도된 탈수초 질환을 가진 생쥐의 척수에서 오스테오폰틴의 면역조직화학적 검출
[Category] 폴리오,
[Article Type] journal-article
[Source] pubmed
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