Abstract
Background: Scientists have been facing numerous challenges in the development of an effective therapeutic strategy for the treatment of COVID-19 pneumonia. Several studies have suggested that improving patient immunity and reducing lung injury induced by SARS-CoV-2 may be effective for treating patients with COVID-19.
Methods: A pilot trial of nebulization therapy with exosomes of mesenchymal stem cells (MSCs) was performed on seven patients with COVID-19 pneumonia. Exosomes secreted from MSCs were collected and purified using multiple ultrafiltration steps. All patients were treated with nebulization of MSC-derived exosomes, and primary safety and efficacy outcomes were evaluated.
Results: Our clinical study demonstrated that nebulization of MSC-derived exosomes is a novel method that might be utilized in the treatment of COVID-19 pneumonia. Nebulization of MSC-derived exosomes did not induce acute allergic or secondary allergic reactions but did promote the absorption of pulmonary lesions and reduce the duration of hospitalization for mild cases of COVID-19 pneumonia.
Conclusions: Nebulization of MSC-derived exosomes is a safe, effective, and simple method, and their application at the beginning of treatment may be more beneficial.
Trial registration: Chinese Clinical Trial Registry, ChiCTR2000030261. Registered on 26 February 2020.
Keywords: COVID-19 pneumonia; Exosomes; Mesenchymal stem cells; Nebulization.
【저자키워드】 COVID-19 pneumonia, Mesenchymal stem cells, Exosomes, nebulization., 【초록키워드】 COVID-19, Treatment, SARS-CoV-2, Exosome, Efficacy, therapy, Trial, Immunity, Pneumonia, MSCs, Lung injury, outcome, clinical, Patient, registry, therapeutic strategy, clinical study, MSC, nebulization, Safe, stem cell, allergic reaction, Chinese, allergic, scientist, duration of hospitalization, effective, Seven, collected, evaluated, was performed, treated, reducing, demonstrated, suggested, promote, induce, reduce, purified, pulmonary lesion, mild case, patients with COVID-19, secreted, 【제목키워드】 COVID-19, Exosome, nebulization, CORD, STEM,