Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2’- O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2’- O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
【저자키워드】 SARS-CoV-2, coronavirus, methyltransferase, nsp10, nsp16, nsp14, capping enzyme, 【초록키워드】 coronavirus disease, severe acute respiratory syndrome coronavirus 2, coronavirus, pandemic, antibody, monoclonal antibody, severe acute respiratory syndrome Coronavirus, RNA, Coronavirus disease-19, nsp10, non-structural protein, respiratory, Coronavirus replication, causative agent, acute respiratory syndrome, subunit, acute respiratory syndrome coronavirus, enzyme, infected cells, complex, component, protein complexes, infected cell, subcellular localization, Cell, investigated, RNA methyltransferase, the SARS-CoV-2, 【제목키워드】 Localization, capping, Revealed,