Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA is detected by reverse-transcription quantitative real-time PCR (RT-qPCR) from respiratory specimens. This study compares throat washings (TW), nasopharyngeal swabs (NS) and oropharyngeal swabs (OS). A total of 102 samples from 34 adult patients with confirmed SARS-CoV-2 infection were analysed by RT-qPCR with absolute quantification. The median concentrations and diagnostic sensitivities were 5.8 × 10 4 copies/mL, 85% (NS), 1.4 × 10 4 , 79% (OS) and 4.3 × 10 3 , 85% (TW). Concentration differences were significant between NS and TW ( P = 0.019). Saliva (SA) was available from 21 patients (median 3.4 × 10 3 ). OS and TW can be considered for SARS-CoV-2 diagnostics, although with slightly lower concentrations.
【저자키워드】 COVID-19, SARS-CoV-2, Saliva, Nasopharyngeal swab, RT-qPCR, PCR, throat washing, oropharyngeal swab, nucleic acid test, diagnostic sensitivity, 【초록키워드】 Saliva, coronavirus, SARS-COV-2 infection, diagnostic, coronavirus 2, diagnostics, RNA, Severe acute respiratory syndrome, sensitivity, RT-qPCR, nasopharyngeal swabs, Patient, respiratory, quantification, Concentration, respiratory specimens, diagnostic sensitivities, acute respiratory syndrome coronavirus 2, oropharyngeal swabs, quantitative real-time PCR, concentrations, analysed, median, 【제목키워드】 detection, Throat,