The recent emergence of SARS-CoV-2 in humans from a yet unidentified animal reservoir and the capacity of the virus to naturally infect pets, farmed animals and potentially wild animals has highlighted the need for serological surveillance tools. In this study, the luciferase immunoprecipitation systems (LIPS), employing the spike (S) and nucleocapsid proteins (N) of SARS-CoV-2, was used to examine the suitability of the assay for antibody detection in different animal species. Sera from SARS-CoV-2 naturally-infected mink ( n = 77), SARS-CoV-2 experimentally-infected ferrets, fruit bats and hamsters and a rabbit vaccinated with a purified spike protein were examined for antibodies using the SARS-CoV-2 N and/or S proteins. From comparison with the known neutralization status of the serum samples, statistical analyses including calculation of the Spearman rank-order-correlation coefficient and Cohen’s kappa agreement were used to interpret the antibody results and diagnostic performance. The LIPS immunoassay robustly detected the presence of viral antibodies in naturally infected SARS-CoV-2 mink, experimentally infected ferrets, fruit bats and hamsters as well as in an immunized rabbit. For the SARS-CoV-2-LIPS-S assay, there was a good level of discrimination between the positive and negative samples for each of the five species tested with 100% agreement with the virus neutralization results. In contrast, the SARS-CoV-2-LIPS-N assay did not consistently differentiate between SARS-CoV-2 positive and negative sera. This study demonstrates the suitability of the SARS-CoV-2-LIPS-S assay for the sero-surveillance of SARS-CoV-2 infection in a range of animal species.
【저자키워드】 SARS-CoV-2, spike, nucleocapsid, mink, animal, luciferase immunoprecipitation systems, sera, 【초록키워드】 antibody, hamsters, neutralization, SARS-COV-2 infection, Human, diagnostic, virus, Spike protein, nucleocapsid protein, immunoassay, Viral, Antibody detection, sera, Virus neutralization, bat, animal reservoir, discrimination, serological surveillance, hamster, rabbit, wild animals, wild animal, Luciferase immunoprecipitation system, LIPS, serum samples, S proteins, Nucleocapsid proteins, statistical analyses, ferrets, calculation, positive, luciferase, SARS-CoV-2 N, viral antibodies, Cohen’s kappa, infect, Spearman, FIVE, immunized, negative sample, tested, was used, examined, were used, purified, statistical analysis, the SARS-CoV-2, viral antibody, 【제목키워드】 detection, Other,