A chemiluminescent-based optical fiber immunosensor was developed for the detection of antipneumococcal antibodies. This was accomplished by developing a different chemical procedure utilizing 3-aminopropyl trimethoxysilane and cyanuric chloride to conjugate pneumococcal cell wall polysaccharides to the optical fiber tips, and by improving the sensitivity of the photodetection system. The lowest titer of antipneumococcal antibodies detected by the optical fiber was at a 1:819,200 dilution. The lowest corresponding value by standard enzyme-linked immunosorbent assay was at a 1:98,415 dilution. It was concluded that the optical immunosensor system is an accurate and sensitive method to detect antipneumococcal antibodies and may be an adequate tool to monitor antibodies in specimens such as saliva and urine.
Development of a Chemiluminescent Optical Fiber Immunosensor to Detect Streptococcus pneumoniae Antipolysaccharide Antibodies
스트렙토코쿠스 뉴모니아 항다당체 항체를 검출하기 위한 화학발광 광섬유 면역센서 개발
[Category] 폐렴구균 감염증,
[Article Type] journal-article
[Source] pubmed
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