We have investigated the fluorescence profiles of four common pathogens: S. pneumoniae, S. aureus, M. catarrhalis, and H. influenzae. The steady-state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combined into a fluorescence profile or fluorescence finger print. Each bacterium produces a unique in vitro fluorescence profile when measured in a saline suspension. The profiles are reproducible. Suspensions of a bacterial strain, where the identification is not known, can be correctly matched to a small library of previously measured fluorescence profiles using a linear least-squares fitting algorithm. In addition, we have measured the fluorescence and absorption spectrum of the tympanic membrane removed from a chinchilla. The optical properties of the tympanic membrane and the least-squares identification process form precept for a non-invasive, fluorescence based bacterial diagnosis technique to be used in otitis media.
Bacteria identification of otitis media with fluorescence spectroscopy
형광 분광법을 이용한 중이염의 세균 식별
[Category] 폐렴구균 감염증,
[Article Type] journal-article
[Source] pubmed
All Keywords