Summary The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest. Graphical abstract Highlights • Quantification of COVID-19 immunoglobulin response • High-throughput analysis of patient cohorts • Multiplexed analysis for IgG, IgA, and IgM • R shiny data analysis software with web-based interface The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.
【저자키워드】 immunology, proteomics, antibody, bioinformatics, Health Sciences, High Throughput Screening, 【초록키워드】 COVID-19, coronavirus disease, IgG, IgM, Coronavirus disease 2019, pandemic, Microarrays, ELISA, Antigen, lateral flow, Immunoglobulin, IgA, Data analysis, antigens, Interaction, Analysis, Abstract, alternative, technique, patient sera, reagent, immunoglobulin response, patient cohorts, patient cohort, responses, variety, antigenic target, 【제목키워드】 COVID-19, protocol, Antigen, Microarray, serological analysis,