Summary One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021) . Graphical abstract Highlights • Cloning strategy allows insertion of SARS-CoV-2 spike gene into pHIV-eGFP • Transfection of transfer and packaging plasmids enables production of viral particles • Cells are transduced to stably express SARS-CoV-2 spike protein on the cell surface • Flow cytometry assay detects specific antibodies against SARS-CoV-2 spike protein One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein.
【저자키워드】 immunology, Molecular biology, antibody, Cell Biology, Health Sciences, Flow Cytometry/Mass Cytometry, 【초록키워드】 COVID-19, public health, coronavirus, protocol, S protein, Transmission, complement, flow cytometry, Spike protein, Viral, Serological assay, serological assays, management, SARS-CoV-2 spike protein, Early detection, specific antibodies, SARS-CoV-2 spike, Viral particles, Plasmid, Cytometry, Flow, the cell, capture, Abstract, cell surface, infected individual, infected individuals, coronavirus 2019 disease, transfer, insertion, Express, transfection, eGFP, full-length SARS-CoV-2 spike protein, PCR-based assays, viral particle, Complete, Cloning, limit, Cell, detect, specific antibody, the S protein, transduced, full-length SARS-CoV-2, patients with COVID-19, 【제목키워드】 serological, anti-spike antibody, COVID-19 patient,