Abstract
Angiotensin-converting enzyme 2 (ACE2) acts as a key receptor for the spike of SARS-CoV-2. Two main microRNAs (miRs), miR-200c-3p and miR-421-5p, are considered to modulate the expression of ACE2 gene and alterations in the expression of these miRNAs may influence the outcomes of COVID-19 infection. Accordingly, we examined whether miRNAs directing ACE2 expression altered in the SARS-CoV-2 infection. 30 patients with COVID-19 included in the study. At the time of admission and discharge, the expression of miR-200c-3p and miR-421-5p, inflammatory cytokine IL-6, and regulatory T cells’ expression profiles (CD4, CD25, and Foxp3) were examined using quantitative real-time PCR method. At the time of admission, the expression levels of miR-200c-3p and miR-421-5p as well as CD4, CD25, and Foxp3 significantly decreased while IL-6 expression notably enhanced. However, by the time of discharge, the expression levels of the genes were opposite to the time of admission. Moreover, Pearson correlation analysis indicated that IL-6 expression negatively correlated with Foxp3 and miR-200c-3p expressions despite miR-421-5p and miR-200c-3p positively correlated at admission time. By manipulating miR-200c-3p and miR-421-5p expressions and controlling the ACE2 level, it is plausible to modulate the inflammation by reducing IL-6 and maintenance tolerance hemostasis during COVID-19 infection.
【저자키워드】 COVID-19, IL-6, ACE-2, miR-200c-3p, miR-421-5p, 【초록키워드】 SARS-CoV-2, Inflammation, Tolerance, ACE2, microRNA, miRNA, Infection, outcome, CD4, discharge, Hemostasis, Regulatory T cell, COVID-19 infection, receptor, ACE2 expression, expression, Admission, CD25, Inflammatory cytokine, Analysis, ACE2 gene, expression level, enzyme, alteration, Pearson correlation, quantitative real-time PCR, IL-6 expression, opposite, expression profile, examined, significantly, indicated, reducing, modulate, correlated, patients with COVID-19, positively correlated, the SARS-CoV-2, 【제목키워드】 immune response, subject,