Abstract
Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
All Keywords
【저자키워드】 Single nucleotide polymorphism, cycle threshold, Next-generation sequencing, Real-time reverse transcription PCR, SNPSingle nucleotide polymorphism, NGSNext-generation sequencing, RT-qPCRreal-time reverse transcription PCR, Ctcycle threshold, 【초록키워드】 Brazil, bioinformatics, Local, Transmission, virus, Nasopharyngeal swab, SARS-CoV-2 genome, Depletion, ribosomal RNA, contributed, 【제목키워드】 COVID-19, Nasopharyngeal swab, Ion,
【저자키워드】 Single nucleotide polymorphism, cycle threshold, Next-generation sequencing, Real-time reverse transcription PCR, SNPSingle nucleotide polymorphism, NGSNext-generation sequencing, RT-qPCRreal-time reverse transcription PCR, Ctcycle threshold, 【초록키워드】 Brazil, bioinformatics, Local, Transmission, virus, Nasopharyngeal swab, SARS-CoV-2 genome, Depletion, ribosomal RNA, contributed, 【제목키워드】 COVID-19, Nasopharyngeal swab, Ion,