Abstract
Introduction
Inflammatory response-induced coagulopathy is a common complication associated with severe form of covid-19 infection. Evidences suggest that neutrophil extracellular traps (NETs) play a significant role in triggering the immunothrombosis in this condition. We aimed to evaluate the diagnostic value of surface neutrophilic myeloperoxidase (MPO) as NETosis biomarker for predicting the risk of covid-19-associated coagulopathies.
Methods
Covid-19 infection was assessed by real-time-PCR and plasma d-dimer levels were measured by ELFA. Based on the covid-19 infection and d-dimer level outcomes, patients were categorized into four groups. Any alteration in the serum level of IL-6, H3Cit and neutrophilic surface MPO were analyzed by CLIA, ELISA, and flow cytometry, respectively.
Results
H3Cit variations and different d-dimer values confirmed the association between NETosis and coagulopathies. Findings showed that the expression of neutrophilic MPO reduced in cases with NETosis, which was correlated with increased levels of H3Cit. ANC/MPO ratio was signified as a valuable marker to discriminate the covid-19 and non covid-19-associated coagulopathies and could be considered as a prognostic factor due to its noteworthy correlation with serum IL-6 concentration.
Conclusion
Declined levels of surface neutrophilic MPO in NETosis correlate with covid-19-associated coagulopathies and increased IL-6 levels, as a potential biomarker of covid-19 disease severity.
【저자키워드】 NETosis, Coagulopathies, Surface myeloperoxidase, 【초록키워드】 CLIA, Biomarker, IL-6, disease severity, Variation, Infection, risk, MPO, flow cytometry, ELISA, immunothrombosis, Coagulopathy, outcomes, serum, Patient, Neutrophil extracellular trap, plasma, correlation, expression, NETs, association, marker, myeloperoxidase, Concentration, Prognostic factor, IL-6 levels, Serum level, diagnostic value, four groups, finding, neutrophilic, triggering, Result, analyzed, evaluate, reduced, correlated, were measured, 【제목키워드】 MPO, Coagulopathy, expression level, neutrophilic, infected patient,