Abstract
Objectives
Observing the serological cross-reactivity between SARS-CoV-2 and dengue virus (DV), we aimed to elucidate its effect on dengue serodiagnosis and infectivity in a highly dengue-endemic city in India.
Methods
A total of 52 COVID-19 (reverse transcription-polymerase chain reaction [RT-PCR] positive) serum samples were tested in rapid lateral flow immunoassays and DV immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) to detect DV or SARS-CoV-2 IgG/immunoglobulin M. The COVID-19 antibody (Ab) positive samples were subjected to a virus neutralization test (Huh7 cells) using DV type 1 (DV1) clinical isolate.
Results
Most (93%) of the SARS-CoV-2 Ab-positive serum samples cross-reacted with DV in rapid or ELISA tests. All were DV RNA and nonstructural protein 1 (NS1) antigen-negative. COVID-19 serum samples that were DV cross-reactive neutralized DV1. Of these, 57% had no evidence of DV pre-exposure (DV NS1 Ab-negative). The computational study also supported potential interactions between SARS-CoV-2 Ab and DV1.
Conclusion
DV serodiagnosis will be inconclusive in areas co-endemic for both viruses. The COVID-19 pandemic appears to impart a protective response against DV in DV-endemic populations.
【저자키워드】 COVID-19, SARS-CoV-2, cross-reactivity, Dengue, Enzyme-linked immunosorbent assay (ELISA), Virus neutralization assay (VNT), Lateral flow immunoassay (LFIA), 【초록키워드】 IgG, COVID-19 pandemic, India, ELISA, RNA, lateral flow immunoassay, Immunoglobulin, virus neutralization test, dengue virus, COVID-19 antibody, serological, Protective, Evidence, Interaction, nonstructural protein 1, cross-reactive, both viruses, positive, HUH7 cells, serum sample, MOST, positive sample, Inconclusive, objective, populations, enzyme-linked immunosorbent, neutralized, Result, tested, detect, supported, appear, had no, Observing, the SARS-CoV-2, 【제목키워드】 neutralization, serum, dengue virus, Neutralizing, cross-reactive,