Abstract
Background
The involvement of SARS-CoV-2 antibodies in mediating immunopathogenetic events in COVID-19 patients has been suggested. By using several experimental approaches, we investigated the potential association between SARS-CoV-2 IgGs recognizing the spike (S) protein receptor-binding domain (RBD), neutralizing antibodies (NtAb) targeting S, and COVID-19 severity.
Patients and methods
This unicenter, retrospective, observational study included 51 hospitalized patients (24 at the intensive care unit; ICU). A total of 93 sera from these patients collected at different time points from the onset of symptoms were analyzed. SARS-CoV-2 RBD IgGs were quantitated by ELISA and NtAb50 titers were measured in a GFP reporterbased pseudotyped virus platform. Demographic and clinical data, complete blood counts, as well as serum levels of ferritin, Dimer-D, C reactive protein (CRP), lactose dehydrogenase (LDH), and interleukin-6 (IL-6) were retrieved from clinical charts.
Results
The overall correlation between levels of both antibody measurements was good (Rho = 0.82; P = 0 < 0.001). SARS-CoV-2 RBD IgG and NtAb50 levels in sera collected up to day 30 after the onset of symptoms were comparable between ICU and non-ICU patients (P=>0.1). Four ICU patients died; two of these achieved NtAb50 titers ≥1/160 while the other two exhibited a 1/80 titer. Very weak (Rho=>0.0–<0.2) or weak (Rho=>0.2–<0.4) correlations were observed between anti-RBD IgGs, NtAb50, and serum levels pro-inflammatory biomarkers.
Conclusions
The data presented herein do not support an association between SARS-CoV-2 RBD IgG or NtAb50 levels and COVID-19 severity.
【저자키워드】 COVID-19, SARS-CoV-2, Neutralizing antibodies, inflammatory biomarkers, 【초록키워드】 neutralizing antibody, IgG, intensive care, antibody, IL-6, COVID-19 severity, interleukin-6, LDH, CRP, ferritin, ELISA, ICU, Protein, SARS-CoV-2 antibody, Pseudotyped virus, RBD, sera, Patient, correlation, demographic, platform, SARS-CoV-2 IgG, Blood, association, retrospective, COVID-19 patient, anti-RBD, SARS-CoV-2 RBD, Support, C reactive protein, Serum level, Clinical data, approaches, domain, IgGs, pro-inflammatory biomarkers, Complete, GFP, ICU patient, event, Result, analyzed, collected, investigated, exhibited, onset of symptom, hospitalized patient, suggested, comparable, recognizing, different time point, were measured, non-ICU patient, retrieved, 【제목키워드】 SARS-CoV-2 antibodies, hospitalized COVID-19 patient, Clinical severity, serum inflammatory biomarker,