The pandemic caused by SARS-CoV-2 resulted in increasing demands for diagnostic tests, leading to a shortage of recommended testing materials and reagents. This study reports on the performance of self-sampled alternative swabbing material (ordinary Q-tips tested against flocked swab and rayon swab), of reagents for classical RNA extraction (phenol/guanidine-based protocol against a commercial kit), and of intercalating dye-based one-step quantitative reverse transcription real-time PCRs (RT-qPCR) compared against the gold standard hydrolysis probe-based assays for SARS-CoV-2 detection. The study found sampling with Q-tips, RNA extraction with classical protocol and intercalating dye-based RT-qPCR as a reliable and comparably sensitive strategy for detection of SARS-CoV-2—particularly valuable in the current period with a resurgent and dramatic increase in SARS-CoV-2 infections and growing shortage of diagnostic materials especially for regions limited in resources.
【저자키워드】 Infectious diseases, Molecular biology, Infectious-disease diagnostics, 【초록키워드】 SARS-CoV-2, pandemic, protocol, SARS-COV-2 infection, diagnostic, RNA extraction, SARS-CoV-2 detection, RT-qPCR, Region, Real-time PCR, diagnostic tests, gold, sampling, reverse transcription, Swab, swabbing, Quantitative, SARS-CoV-2 infections, guanidine, phenol, gold standard, reagents, reagent, dramatic increase, resources, hydrolysis, tested, classical, caused, 【제목키워드】 SARS-CoV-2, Diagnosis,