Abstract
Background
Respiratory tract infections (RTIs) represent a major cause of clinical visits worldwide. Viral epidemiology of RTIs in adults has been less studied compared to children. FilmArray respiratory panel (FA-RP), a multiplex, real time polymerase chain reaction method can simultaneously detect the nucleic acids of multiple pathogens. The purpose of this study is to analyze the epidemiology and clinical presentations of an RTI cohort.
Methods
This retrospective cohort study was conducted at China Medical University Hospital (CMUH) and China Medical University Children’s Hospital (CMUCH), from January 2020 to June 2020. The FA-RP results were collected and analyzed according to upper versus lower RTIs.
Results
Among 253 respiratory samples tested, 135 (53.4%) were from adults and 118 (46.6%) from children. A total positive rate of 33.9% (86/253) was found, with 21.48% (29/135) in adults and 48.31% (57/118) in children. Human rhinovirus/Enterovirus (HRV/EV) was detected in most of the age groups and was more common in URIs. HRV/EV was found as a frequent co-detection virus. Among children, HRV/EV was the most detected pathogen of URIs, while the most predominant pathogen in LRIs was Mycoplasma pneumoniae.
Conclusions
FA-RP has the potential to improve the detection rate of respiratory pathogens. The positive rate of FA-RP was higher in children compared to adults, which likely corresponds to the higher incidence of viral RTIs in children. Different pathogens may lead to different types of respiratory infections.
【저자키워드】 Multiplex PCR, respiratory tract infection, virus, Atypical pathogen, 【초록키워드】 respiratory infections, Epidemiology, children, Human, Infection, China, nucleic acid, Adults, Cohort, pathogen, Respiratory pathogens, age, Pathogens, incidence, respiratory, multiplex, group, university, detection rate, positive rate, Mycoplasma pneumoniae, clinical presentation, real time, retrospective cohort study, respiratory sample, polymerase chain, IMPROVE, Result, tested, analyzed, detect, collected, conducted, less, predominant, 【제목키워드】 COVID-19 pandemic, hospital, detection, Taiwan, respiratory pathogen,