Abstract
SARS-CoV-2 has caused COVID-19 pandemic globally in the beginning of 2020, and qualitative real-time RT-PCR has become the gold standard in diagnosis. As SARSCoV-2 with strong transmissibility and pathogenicity, it has become a professional consensus that clinical samples from suspected patients should be heat inactivated at 56°C for 30 min before further processing. However, previous studies on the effect of inactivation on qualitative real-time RT-PCR were conducted with diluted samples rather than clinical samples. The aim of this study was to investigate whether heat inactivation on clinical samples before detection will affect the accuracy of qualitative real-time RT-PCR detection. All 46 throat swab samples from 46 confirmed inpatients were detected by qualitative real-time RT-PCR directly, as well as after heat inactivation. Heat-Inactivation has significantly influenced the qualitative detection results on clinical samples, especially weakly positive samples. The results indicate the urgency to establish a more suitable protocol for COVID-19 clinical sample’s inactivation.
【저자키워드】 COVID-19, SARS-CoV-2, RNA detection, heat-inactivation, Qualitative real-time RT-PCR, 【초록키워드】 protocol, COVID-19 pandemic, Diagnosis, clinical samples, Transmissibility, Accuracy, inactivation, heat inactivation, real-time RT-PCR, pathogenicity, inactivated, SARSCoV-2, Inpatient, Consensus, gold standard, urgency, previous study, positive, throat swab, qualitative detection, Affect, clinical sample, caused, significantly, conducted, suspected patient, 【제목키워드】 inactivation, real-time RT-PCR, detection rate, Cycle threshold value, heat, decrease, clinical sample,