Abstract
Nitrocellulose (NC) membrane can have value-added applications for lateral flow assay (LFA)-based diagnostic tools, which has great potential for the detection of pathogens, such as COVID-19, in different environments. However, poor sensitivity of the NC membrane based LFA limits its further application in many cases. Herein, we developed a facile method for LFA sensitivity enhancement, by incorporating two-sugar barrier into LFAs: one between the conjugation pad and the test line, and the other between the test line and the control line. ORF1ab nucleic acid of COVID-19 was used as the model target to demonstrate the concept on the HF120 membrane. Results show that at optimum conditions, the two sugar barrier LFAs have a detection limit of 0.5 nM, which is compared to that of 2.5 nM for the control LFA, achieving a 5-fold sensitivity increase. This low cost, easy-to-fabricate and easy-to-integrate LFA method may have potential applications in other cellulose paper-based platforms.
【저자키워드】 COVID-19, Lateral flow assay (LFA), Nitrocellulose membrane, HF120, Sensitivity enhancement, Bio-recognition event, 【초록키워드】 diagnostic, lateral flow, nucleic acid, sensitivity, membrane, Pathogens, Cellulose, Detection limit, ORF1ab, sugar, nitrocellulose, many cases, test line, limit, Result, was used, conditions, control line, NC membrane, 【제목키워드】 lateral flow, nucleic acid, sensitivity, sugar, ENhance,