[[[ Objective: ]]] To research the pathogenicity of Salmonella Pathogenicity Island 2 (SPI-2) deletion mutant of Salmonella Pullorum and preliminary explore the feasibility of developing safe attenuated Salmonella Pullorum candidate vaccine strain. [[[ Methods: ]]] The SPI-2 (-40 kb) deletion mutant of Salmonella Pullorum S06004 was constructed using the λ-red recombinant system. Then the biological characteristics such as growth rate, biochemical properties, genetic stability and virulence were evaluated between the deletion mutant strain S06004ΔSPI2 and its parent strain S06004. [[[ Results: ]]] S06004ΔSPI2 was successfully constructed. The growth rate and biochemical properties of S06004ΔSPI2 were consistent with those of its parent strain S06004. The mutant was stable with the deletion of SPI-2. Chicken lethal test showed that the LD50 of S06004ΔSPI2 was 252 times higher than the parent strain S06004. [[[ Conclusion: ]]] The virulence of S06004ΔSPI2 was obviously attenuated. This study provided basic data for further study of the functions of SPI-2, and implied its potential to develop attenuated Salmonella vaccine.
[Construction and characterization of Salmonella Pathogenicity Island 2 (SPI-2) deletion mutant of Salmonella Pullorum S06004]
[살모넬라 풀로룸 S06004의 살모넬라 병원성 섬 2 (SPI-2) 결실 변종의 구축 및 특성화]
[Category] 살모넬라증,
[Source] pubmed
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