Infection with SARS-CoV-2, the Betacoronavirus, caused a pandemic that affected the globe negatively. The gold method, RT-PCR, can detect SARS-CoV-2 but it is time-consuming and needs sophisticated equipment and professional personnel. On the other hand, rapid tests offer fast results and can detect anti-SARS-CoV-2 antibodies (Abs). The aim of this study is to develop a new rapid and cost-effective method for the detection of anti-SARS-CoV-2 IgG/IgM Abs. A new top-loading detection device was developed and composed of a small piece of plastic (25 × 25 × 0.5 mm) with an opening in the center, a piece of nitrocellulose (NC) membrane enough to block the opening from one side and adhesive tape to affix the NC to the plastic piece. The NC is blotted with anti-human IgG/IgM and rabbit serum. The device was evaluated against a commercially available IgG/IgM ELISA detection kit using normal, Covid-19-positive, HCV, HBV, and Cytomegalovirus-positive sera. Outcomes demonstrated simplicity, reproducibility, and accuracy of the new device and results can be obtained in less than 5 min. We anticipate our developed assay method to be used widely in point of care before deciding on the use of expensive nucleic acid assays.
【저자키워드】 Immunological techniques, Biological techniques, Biological models, 【초록키워드】 SARS-CoV-2, pandemic, equipment, point of care, RT-PCR, anti-SARS-CoV-2, Betacoronavirus, HCV, ELISA, HBV, nucleic acid, serum, anti-SARS-CoV-2 antibodies, Rapid test, anti-SARS-CoV-2 antibody, Accuracy, anti-SARS-CoV-2 IgG, plastic, sera, gold, membrane, cytomegalovirus, Rapid tests, Device, opening, adhesive tape, nitrocellulose, center, simplicity, piece, reproducibility, anti-human IgG, offer, affected, detect, develop, caused, globe, assays, composed, evaluated, less, demonstrated, time-consuming, the Betacoronavirus, 【제목키워드】 anti-SARS-CoV-2, development, IgG/IgM antibody,