Serological testing to evaluate antigen-specific antibodies in plasma is generally performed by rapid lateral flow test strips that lack quantitative results or by high complexity immunoassays that are time- and labor-intensive but provide semi-quantitative results. Here, we describe a novel application of biolayer interferometry for the rapid detection of antigen-specific antibody levels in plasma samples, and demonstrate its utility for quantification of SARS-CoV-2 antibodies. Our biolayer interferometry immunosorbent assay (BLI-ISA) utilizes single-use biosensors in an automated “dip-and-read” format, providing real-time optical measurements of antigen loading, plasma antibody binding, and antibody isotype detection. Complete semi-quantitative results are obtained in less than 20 min. BLI-ISA meets or exceeds the performance of high complexity methods such as Enzyme-Linked Immunosorbent Assay (ELISA) and Chemiluminescent Immunoassay. Importantly, our method can be immediately implemented on existing BLI platforms for urgent COVID-19 studies, such as serosurveillance and the evaluation of vaccine candidates. In a broader sense, BLI-ISA can be developed as a novel diagnostic platform to evaluate antibodies and other biomolecules in clinical specimens.
【저자키워드】 antibodies, Immunological techniques, Applied immunology, Infectious-disease diagnostics, 【초록키워드】 COVID-19, antibody, diagnostic, ELISA, Antigen, immunoassay, test strips, enzyme-linked immunosorbent assay, SARS-CoV-2 antibodies, Lateral flow test, Serological testing, plasma, automated, optical, chemiluminescent immunoassay, utility, quantification, Quantitative, specimens, platform, binding, enzyme, plasma samples, plasma antibody, vaccine candidates, labor, antigen-specific antibody, BLI, performed, lack, evaluate, less, semi-quantitative, immunosorbent, labor-intensive, meet, 【제목키워드】 SARS-CoV-2 antibody, Rapid,