Abstract
α-Glucosidase is a promising target for the treatment of diabetes. Drug repurposing can increase the chances of discovering an active inhibitor. Therefore, this study aimed to identify potential α-glucosidase inhibitor using drug repurposing and in silico strategies. We identified critical amino acid residues of the three α-glucosidase proteins. Based on cross molecular docking studies of three α-glucosidase proteins and drugs in the FDA database, we screened hits with the favorable binding affinities and modes targeting the three proteins. Subsequently, an in vitro activity assay showed that raloxifene was an excellent inhibitor of α-glucosidase. Moreover, molecular dynamics simulations of raloxifene and three proteins were performed to assess the stability of the protein-hit systems in physiological conditions and clarify protein-hit interactions. We also performed the binding free energy calculation, Hirshfeld surface and alanine scanning mutagenesis analyses. These results demonstrated that binding between raloxifene and the three proteins was stable, and the critical amino acid residues of the three proteins formed stable contacts with raloxifene. The molecular mechanisms agree well with its activity, reinforcing that raloxifene is a candidate α-glucosidase inhibitor. Our study smoothes the path for the development of novel a-glucosidase inhibitors with high efficacy and low toxicity for the treatment of diabetes.
【저자키워드】 Drug repurposing, Virtual screening, molecular dynamics simulations, raloxifene, α-Glucosidase inhibitor, Cross molecular docking, 【초록키워드】 Treatment, Efficacy, Toxicity, Proteins, drug, diabetes, in silico, molecular mechanism, FDA, binding free energy, database, binding affinity, Molecular dynamics simulation, Protein, stability, cross, inhibitor, Critical, interactions, binding, Mutagenesis, in vitro activity, Contact, Molecular docking study, Alanine, amino acid residue, identify, performed, screened, demonstrated, analyses, physiological condition, 【제목키워드】 cross, molecular, inhibitor, Combination, identification, approach,