Abstract
The SARS-CoV-2 virus emerged in December 2019 and has caused a worldwide pandemic due to the lack of any pre-existing immunity. Accurate serology testing is urgently needed to help diagnose infection, determine past exposure of populations and assess the response to a future vaccine. The landscape of antibody responses to SARS-CoV-2 is unknown. In this study, we utilized the luciferase immunoprecipitation system to assess the antibody responses to 15 different SARS-CoV-2 antigens in patients with COVID-19. We identified new targets of the immune response to SARS-CoV-2 and show that nucleocapsid, open reading frame (ORF)8 and ORF3b elicit the strongest specific antibody responses. ORF8 and ORF3b antibodies, taken together as a cluster of points, identified 96.5% of COVID-19 samples at early and late time points of disease with 99.5% specificity. Our findings could be used to develop second-generation diagnostic tests to improve serological assays for COVID-19 and are important in understanding pathogenicity.
【초록키워드】 COVID-19, antibodies, SARS-CoV-2, Vaccine, immune response, antibody, Antibody Response, diagnostic test, Infection, SARS-CoV-2 virus, Population, specificity, Serological assay, ORF8, nucleocapsid, serology testing, Cluster, target, SARS-CoV-2 antigen, pathogenicity, disease, pre-existing immunity, diagnose, Luciferase immunoprecipitation system, Frame, worldwide pandemic, help, ORF3b, responses, IMPROVE, lack, develop, caused, determine, elicit, the antibody response, COVID-19 sample, patients with COVID-19, 【제목키워드】 antibody, SARS-COV-2 infection, ORF8, ORF3b, serological marker,