Abstract
We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase–receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.
All Keywords
【초록키워드】 neutralizing antibody, SARS-CoV-2, coronavirus, neutralization, variant, virus, angiotensin-converting enzyme 2, binding affinity, ACE-2, Protein, serum, RBD, binding, acute respiratory syndrome, domain, Modification, approach, detect, assays, thermodynamic, 【제목키워드】 neutralizing antibody, SARS-CoV-2 variant,
【초록키워드】 neutralizing antibody, SARS-CoV-2, coronavirus, neutralization, variant, virus, angiotensin-converting enzyme 2, binding affinity, ACE-2, Protein, serum, RBD, binding, acute respiratory syndrome, domain, Modification, approach, detect, assays, thermodynamic, 【제목키워드】 neutralizing antibody, SARS-CoV-2 variant,