Abstract
The cytokine interleukin 6 (IL-6) is involved in the pathogenesis of different inflammatory diseases, including cancer, and its monitoring could help diagnosis, prognosis of relapse-free survival and recurrence. Here, we report an innovative microfluidic approach that uses the fluidization of magnetic beads to specifically extract, preconcentrate and fluorescently detect IL-6 directly on-chip. We assess how the physical properties of the beads can be tuned to improve assay performance by enhancing mass transport, reduce non-specific binding and multiply the detection signal threefold by transitioning between packed and fluidization states. With the integration of a full ELISA protocol in a single microfluidic chamber, we show a twofold reduction in LOD compared to conventional methods along with a large dynamic range (10 pg/mL to 2 ng/mL). We additionally demonstrate its application to IL-6 detection in undiluted serum samples.
【초록키워드】 Inflammatory diseases, Pathogenesis, Prognosis, IL-6, Cancer, Diagnosis, cytokine, survival, non-specific, binding, Transport, serum samples, help, magnetic bead, approach, physical, IMPROVE, detect, involved, reduce, reduction in, ELISA protocol, 【제목키워드】 Biomarker, IL-6, cytokine,