Abstract
The ongoing COVID-19 pandemic has caused a global economic and health crisis. To identify host factors essential for coronavirus infection, we performed genome-wide functional genetic screens with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human coronavirus 229E. These screens uncovered virus-specific as well as shared host factors, including TMEM41B and PI3K type 3. We discovered that SARS-CoV-2 requires the lysosomal protein TMEM106B to infect human cell lines and primary lung cells. TMEM106B overexpression enhanced SARS-CoV-2 infection as well as pseudovirus infection, suggesting a role in viral entry. Furthermore, single-cell RNA-sequencing of airway cells from patients with COVID-19 demonstrated that TMEM106B expression correlates with SARS-CoV-2 infection. The present study uncovered a collection of coronavirus host factors that may be exploited to develop drugs against SARS-CoV-2 infection or future zoonotic coronavirus outbreaks.
【초록키워드】 SARS-CoV-2, Coronavirus infection, coronavirus, SARS-COV-2 infection, COVID-19 pandemic, Genetic, drug, airway, Protein, Outbreaks, Health, Factors, expression, Tmem41b, acute respiratory syndrome, Factor, Zoonotic coronavirus, PI3K, pseudovirus infection, Single-cell RNA-sequencing, overexpression, lung cells, human coronavirus 229E, infect, Host, Cell, identify, performed, develop, caused, functional, in viral, demonstrated, lysosomal, human cell line, patients with COVID-19, TMEM106B, 【제목키워드】 SARS-CoV-2, CRISPR, Host, identify, TMEM106B,