Abstract
The first ever US Food and Drug Administration-approved messenger RNA vaccines are highly protective against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)1,2,3. However, the contribution of each dose to the generation of antibodies against SARS-CoV-2 spike (S) protein and the degree of protection against novel variants warrant further study. Here, we investigated the B cell response to the BNT162b2 vaccine by integrating B cell repertoire analysis with single-cell transcriptomics pre- and post-vaccination. The first vaccine dose elicits a recall response of IgA+ plasmablasts targeting the S subunit S2. Three weeks after the first dose, we observed an influx of minimally mutated IgG+ memory B cells that targeted the receptor binding domain on the S subunit S1 and likely developed from the naive B cell pool. This response was strongly boosted by the second dose and delivers potently neutralizing antibodies against SARS-CoV-2 and several of its variants.
【초록키워드】 neutralizing antibody, SARS-CoV-2, Vaccine, BNT162b2 vaccine, coronavirus, antibody, variant, transcriptomics, drug, variants, Receptor binding domain, B cell, vaccine dose, Protein, recall, single-cell, memory B cell, Protective, SARS-CoV-2 spike, food, Analysis, dose, B cell response, Messenger RNA, acute respiratory syndrome, subunit, second dose, first dose, plasmablast, investigated, elicit, mutated, 【제목키워드】 BNT162b2 vaccine, B cell response, SARS-CoV-2 S1, induce,