Angola was the main origin country for the imported malaria in Henan Province, China. Antimalarial drug resistance has posed a threat to the control and elimination of malaria. Several molecular markers were confirmed to be associated with the antimalarial drug resistance, such as pfcrt , pfmdr1 , pfdhfr , pfdhps , and K13. This study evaluated the drug resistance of the 180 imported Plasmodium falciparum isolates from Angola via nested PCR using Sanger sequencing. The prevalences of pfcrt C_{72}V_{73}M_{74}N_{75}K_{76}, pfmdr1 N_{86}Y_{184}S_{1034}N_{1042}D_{1246}, pfdhfr A_{16}N_{51}C_{59}S_{108}D_{139}I_{164}, and pfdhps S_{436}A_{437}A_{476}K_{540}A_{581} were 69.4%, 59.9%, 1.3% and 6.3%, respectively. Three nonsynonymous (A578S, M579I, and Q613E) and one synonymous (R471R) mutation of K13 were found, the prevalences of which were 2.5% and 1.3%, respectively. The single nucleotide polymorphisms (SNPs) in pfcrt , pfmdr1 , pfdhfr , and pfdhps were generally shown as multiple mutations. The mutant prevalence of pfcrt reduced gradually, but pfdhfr and pfdhps still showed high mutant prevalence, while pfmdr1 was relatively low. The mutation of the K13 gene was rare. Molecular surveillance of artemisinin (ART) resistance will be used as a tool to evaluate the real-time efficacy of the artemisinin-based combination therapies (ACTs) and the ART resistance situation.
【저자키워드】 Angola, drug resistance, Plasmodium falciparum, K13, Pfcrt, Pfmdr1, Pfdhfr, Pfdhps,