Abstract
Non-human primates are attractive laboratory animal models that accurately reflect both developmental and pathological features of humans. Here we present a compendium of cell types across multiple organs in cynomolgus monkeys (Macaca fascicularis) using both single-cell chromatin accessibility and RNA sequencing data. The integrated cell map enables in-depth dissection and comparison of molecular dynamics, cell-type compositions and cellular heterogeneity across multiple tissues and organs. Using single-cell transcriptomic data, we infer pseudotime cell trajectories and cell-cell communications to uncover key molecular signatures underlying their cellular processes. Furthermore, we identify various cell-specific cis-regulatory elements and construct organ-specific gene regulatory networks at the single-cell level. Finally, we perform comparative analyses of single-cell landscapes among mouse, monkey and human. We show that cynomolgus monkey has strikingly higher degree of similarities in terms of immune-associated gene expression patterns and cellular communications to human than mouse. Taken together, our study provides a valuable resource for non-human primate cell biology.
【초록키워드】 animal model, heterogeneity, Laboratory, humans, trajectory, transcriptomic data, molecular, non-human primate, single-cell, cellular, similarity, cell type, organs, valuable resource, chromatin, element, cellular processes, RNA sequencing data, single-cell level, feature, Cell, gene regulatory network, identify, provide, analysis, developmental, gene expression pattern, multiple organ, multiple tissue, Non-human, organ-specific, 【제목키워드】 single-cell, transcriptomic,