Abstract
Respiratory syncytial virus is a leading cause of morbidity and mortality in children, due in part to their distinct immune system, characterized by impaired induction of Th 1 immunity. Here we show application of cationic adjuvant formulation CAF08, a liposomal vaccine formulation tailored to induce Th 1 immunity in early life via synergistic engagement of Toll-like Receptor 7/8 and the C-type lectin receptor Mincle. We apply quantitative phosphoproteomics to human dendritic cells and reveal a role for Protein Kinase C-δ for enhanced Th1 cytokine production in neonatal dendritic cells and identify signaling events resulting in antigen cross-presentation. In a murine in vivo model a single immunization at birth with CAF08-adjuvanted RSV pre-fusion antigen protects newborn mice from RSV infection by induction of antigen-specific CD8 + T-cells and Th1 cells. Overall, we describe a pediatric adjuvant formulation and characterize its mechanism of action providing a promising avenue for development of early life vaccines against RSV and other respiratory viral pathogens.
【초록키워드】 Vaccine, Immunity, pediatric, children, Infection, immune system, virus, CD8, immunization, Antigen, Newborn, RSV, mice, Pathogens, morbidity and mortality, receptor, T-cell, Phosphoproteomics, respiratory, mechanism of action, Quantitative, dendritic cell, Signaling, Neonatal, kinase, pre-fusion, murine, Th1 cells, synergistic, event, respiratory viral, Th1 cytokine, PROTECT, resulting, identify, characterized, induce, cationic, in vivo model, 【제목키워드】 Infection, Newborn, respiratory syncytial virus, single dose, murine,